ABSTRACT
Objective To explore the diagnostic value of combined detection of CMV-IgM and CMVDNA in infantile cytomegalovirus hepatitis.Methods The serum CMV virus antibodies of 122 children diagnosed of cytomegalovirus hepatitis in Liaocheng People's Hospital was detected by chemiluminescence,while using fluorescent Probe PCR assay with serum and urine CMV-DNA,The other patients from the health examination center with no infection in children were subject to control group.The differences of CMV antibody and CMV-DNA positive rate were analyzed in infant of cytomegalovirus hepatitis.The diagnostic value of single and combined CMV-IgM and CMV-DNA in infant cytomegalovirus hepatitis was compared by ROC curve.Results Compared with the control group,There were significant differences in the positive rates of CMV-IgM and CMV-DNA (P＜0.01).According to the age group,the total positive rate of 1 days to 6 months group combined with CMV-IgM and CMV-DNA in the highest;clinical features of grouping,the total positive rate of CMV-IgM and CMV-DNA the highest jaundice group,the differences were statistically significart (P ＜0.05).Through the ROC curve analysis,the curve of the joint detection of CMV-IgM and CMV-DNA in the hepatitis group was the largest,and the sensitivity,specificity,positive predictive value and negative predictive value were the highest,and the diagnostic value was the highest.Conclusion Combined with CMV-IgM and CMV-DNA detection in infant of cytomegalovirus hepatitis has a better diagnostic value.
ABSTRACT
Objective To explore the clinical value of combined detection of human leucocyte antigen G (HLA-G) and interleu-kin10(IL-10) in children patients with cytomegalovirus(CMV) hepatitis .Methods 122 children patients with CMV hepatitis in our hospital from January 2013 to January 2017 were selected as the children patients group and contemporaneous 116 healthy children were selected as the control group .The levels of plasma HLA-G and IL-10 were detected by adopting ELISA .Then their correlation and diagnostic value were analyzed .Results The levels of plasma HLA-G and IL-10 in the children patients group were significant-ly higher than those in the control group ,and the difference was statistically significant (P<0 .05) .In grouping according to the age of the month ,the levels of plasma HLA-G and IL-10 in the 1-6 months old patients group were highest .HLA-G and IL-10 had a positive correlation(r=0 .445) .Moreover ,the receiver operating characteristic curve analysis results showed that the area under the curve of combined detection of plasma HLA-G and IL-10 was maximal and its diagnostic efficiency was highest .Conclusion The combined detection of plasma HLA-G and IL-10 can increase the accuracy of CMV infection diagnosis and has an important clinical significance .